The player and the Uruguay FA launched an appeal against the ban, but have had the appeal rejected by FIFA's appeals committee.
FIFA head of media Delia Fischer told a news conference in Rio de Janeiro: "The FIFA appeal committee has decided to reject the appeals lodged by both the Uruguayan player Luis Suarez and the Uruguayan FA, and to confirm the decision rendered by the FIFA disciplinary committee on June 25 2014 in its entirety.
"The terms of the decision taken by the FIFA appeal committee were communicated to the player and the Uruguayan FA today."
Both the player and the AUF can make a further appeal to the Court of Arbitration for Sport.
Suarez has picked up lengthy bans in each of the last three seasons, with two for biting and one for racially abusing Patrice Evra.
Yet he remains one of the most sought-after players on the planet, and Barcelona are reportedly on the verge of agreeing a £75 million purchase of the player from Liverpool.
- Sports & Recreation
- Luis Suarez