Pietersen made 19 against India A in his first limited-overs appearance since reversing his retirement, but was not given the chance to improve on that at the Delhi Daredevils ground.
England brought in captain Alastair Cook, Joe Root and Stuart Meaker, with Jos Buttler and Tim Bresnan missing out alongside Pietersen.
Cook missed the 53-run defeat to India A on Sunday due to a cold but was back to lead the side against Delhi.
After choosing to bat first, Cook made 44 before he was first man out with the score at 70.
Parvinder Awana almost dismissed Cook in the first over but the left-hander was fortunate to see the ball land safe after cannoning off bat and pad.
The first boundary of the day arrived in the fourth over when Cook nicked Ashish Nehra to third man and the runs soon started to add up, four more off the pads and a straight drive in the same over getting the ball rolling.
Ian Bell, who made 91 in the previous match, was going somewhat slower at the other end as Cook scored 28 of England's 43 runs at the 10-over mark.
Bell found his touch with a wonderful lofted four over extra-cover as England passed 50 in 71 balls and was 21 not out after 15 overs.
Cook fell in the 16th over, clean bowled by Sumeet Narwal after a 58-ball stay including seven fours.
- Sports & Recreation
- Alastair Cook
- Delhi Daredevils