The 32-year old playmaker, who last played for the Czechs against Greece at Euro 2012 where he injured his Achilles', has also been troubled by a calf injury this season and returned for club side Arsenal at the weekend.
"I saw Tomas in the FA Cup at the weekend and he has lost none of his qualities," coach Michal Bilek told reporters.
The Czechs will be without ill midfielder Tomas Huebschmann, and injured midfielder Vaclav Pilar and forward Tomas Necid. Viktoria Plzen forward Stanislav Tecl has won his first call-up.
The Czechs are third in World Cup qualifying Group B and face Denmark and Armenia in March.