The older Tunnicliffe placed a £100 bet on his son representing United when he was only nine and with odds of 100-1 he earned £10,000 when the midfielder came on as a substitute after 77 minutes.
He also placed another £100 bet on Tunnicliffe playing for England's senior team at odds of 350/1, possibly earning him £35,000 from William Hill.
Tunnicliffe, now 19, played 30 times during a loan spell with Peterborough United last season but the player who came through the ranks at Carrington had to wait until last night to finally pull on the famous red shirt.
United won 2-1 at home to Newcastle on a special night for the Tunnicliffe family.
The successful bet is reminiscent of that placed by the father of Chris Kirkland, who also won £10,000 when the goalkeeper made his England debut in August 2006.
Eddie Kirkland placed a £100 bet at odds of 100-1 that his son, then around 14, would represent his national side.
It was the only cap the former Wigan and Liverpool goalkeeper ever won.