The young striker was given a red card after the match with Napoli on Sunday, and will be suspended for the game against Bologna on Wednesday.
“Mario has made a mistake, penalizing the team, club and the fans and he must change this,” he told a press conference.
“Balotelli should help referees to protect him because if another player had suffered the fouls he did on Sunday then they would have whistled. The referees should be left alone because they can be influenced by negative attitudes.
“Players must think just about playing and not think about the decisions of the referees. There are situations that go beyond the rules of good manners. When the match is over it is over. Balotelli was wrong and that's that, he must grow-up. He is a world-class player and an Italy international so he should set an example. He has been fine up until Sunday so hopefully he has learnt his lesson.
“Mario is 23-years-old, he is no longer a child and to be a champion you have to have a correct behaviour because you are an example for all those who look up to you. I hope for him he benefits from it and realizes that put team and club is in difficulty.
“You have to go on the pitch thinking about doing well. When you finish the game you need to go in the dressing room, accept what happened and think of working to do better. The club will decide what to do about Mario."
Balotelli will also miss Milan's Serie A matches against Sampdoria and reigning champions Juventus.