Head coach Scott Johnson has opted to give Chris Fusaro his first cap at openside flanker in place of Brown following Sunday's 28-6 loss to Ireland in Dublin.
"I'm absolutely delighted. Being the Calcutta Cup, there's no better game to get your first cap. I'm still pinching myself a bit," Fusaro told the Scottish Rugby Football Union's website (www.scottishrugby.org).
Johnson said the 24-year-old Fusaro was picked to play a certain role.
"England are powerful, they have an edge to their side and are assertive and aggressive," the coach said. "They will probably consider themselves unlucky last weekend but it's not always brute force that wins these games."
England had victory snatched from their grasp in the dying minutes in Paris last weekend, losing 26-24 to France.
Scrum-half Greig Laidlaw will captain Scotland for the fourth time while the two other changes involve Tommy Seymour coming in for the injured Sean Maitland on the right wing and Matt Scott taking Duncan Taylor's place at centre.
Taylor is one of three changes on the bench with hooker Scott Lawson preferred to Pat MacArthur and lock Jonny Grey winning the nod from brother Richie.
Scotland team to face England
15-Stuart Hogg, 14-Tommy Seymour, 13-Alex Dunbar, 12- Matt Scott, 11-Sean Lamont, 10-Duncan Weir, 9-Greig Laidlaw (captain); 8-David Denton, 7-Chris Fusaro, 6-Ryan Wilson, 5-Jim Hamilton, 4-Tim Swinson, 3-Moray Low, 2-Ross ford, 1-Ryan Grant
Replacements: 16-Scott Lawson, 17-Alasdair Dickinson, 18-Geoff Cross, 19-Jonny Gray, 20-Johnnie Beattie, 21-Chris Cusiter, 22- Duncan Taylor, 23-Max Evans
England team to face Scotland
15-Mike Brown, 14-Jack Nowell, 13-Luther Burrell, 12-Billy Twelvetrees, 11-Jonny May, 10-Owen Farrell, 9-Danny Care; 8-Billy Vunipola, 7-Chris Robshaw (captain), 6-Tom Wood, 5-Courtney Lawes, 4-Joe Launchbury, 3-Dan Cole, 2-Dylan Hartley, 1-Joe Marler.
Replacements: 16-Tom Youngs, 17-Mako Vunipola, 18-Henry Thomas, 19-Dave Attwood, 20-Ben Morgan, 21-Lee Dickson 22-Brad Barritt, 23-Alex Goode.