Italian teams Androni Giocattoli, Bardiani Valvole-CSF Inox and Vini Fantini plus Colombia will join the 18 UCI Pro Teams in the opening Grand Tour of the season.
The UCI rejected Katusha's application to compete in the World Tour because of the team's doping record over the past four years.
Katusha, who finished second in the World Tour standings in 2012, have appealed the decision to the Court of Arbitration for Sport.
It means world number one Joaquim Rodriguez, who won two stages in the 2012 race when finishing second overall to Canadian Ryder Hesjedal, will not be able to bid for the Maglia Rosa.
RCS Sport said in a statement: "In order to respect the teams, athletes, media and crucially, the fans, RCS Sport have made the decision to allocate the Wild Card places today, despite the current uncertainty regarding World Tour licences.
"Russian Team Katusha’s position has been judged as it stands today - a Professional Continental Team."
The three-week race begins in Naples on May 4.
UCI Pro Team (invited by right):
AG2R LA MONDIALE (FRA)
ASTANA PRO TEAM (KAZ)
BMC RACING TEAM (USA)
EUSKALTEL – EUSKADI (SPN)
FDJ – BIG MAT (FRA)
FORMER RABOBANK (NED)
GARMIN – SHARP (USA)
LAMPRE – MERIDA (ITA)
LOTTO BELISOL (BEL)
MOVISTAR TEAM (SPN)
OMEGA PHARMA – QUICKSTEP CYCLING TEAM (BEL)
ORICA - GREENEDGE (AUS)
RADIOSHACK – NISSAN (LUX)
SKY PROCYCLING (GBR)
TEAM ARGOS – SHIMANO (NED)
TEAM SAXO - TINKOFF (DEN)
VACANSOLEIL – DCM PRO CYCLING TEAM (NED)
UCI Professional Team (Wild Card):
ANDRONI GIOCATTOLI (ITA) – Automatic right to participate as winner of the Coppa Italia 2012
BARDIANI VALVOLE – CSF INOX (ITA)
VINI FANTINI (ITA)