Villas-Boas worked for Mourinho at Porto, Inter Milan and Chelsea but the friendship has broken down, with the Spurs boss insisting it is no longer a concern ahead of the first contest between the pair as managers.
Mourinho said: "I don't describe (the relationship), because I'm not a kid to discuss relationships with the media. It's a personal thing.
"I don't care what he says. I'm here, not to comment on what he says or what to know what he says. I'm not interested."
However, there was an awkward conclusion to his pre-match media conference when Mourinho was asked if the "spirit" of Sir Bobby Robson, cited by both men as a mentor, would be at White Hart Lane.
Mourinho said: "Why? Where (did) he work with Andre?"
Mourinho was repeatedly asked about Villas-Boas, but time and again refused to respond to his inquisitors.
"It's enough from me," Mourinho added.
"I have nothing to say, nothing at all.
"I'm not discussing here in front of you. For me there is no point.
"I'm not disappointed. I'm just here to speak about anything you want related to the game, not related to this situation."
Mourinho, aged 50, is 15 years the senior of Villas-Boas, who had an unhappy nine-month spell at Chelsea after leading Porto to a Portuguese League and Cup double, as well as the Europa League title.
The Chelsea boss insists he is open with all his assistants and that it is up to them whether they learn from his methods.
When they come into direct opposition Mourinho insists it is important to be professional, like he was in the 2010 Champions League final, when his Inter side beat Luis van Gaal's Bayern Munich.
"I've had so many assistants in my career," said Mourinho, who worked with Van Gaal at Barcelona.
"I was always an open book to all of them. I am an open book, after that if they want to read the book, or not, it's their problem, not my problem."
Asked if he will meet with Villas-Boas, perhaps over a glass of Portuguese red wine, following Saturday's match, Mourinho insisted he would not turn an invitation down.
"When people invite me, I always go," he said.
"I never refuse."