"Mario Balotelli is suffering an injury to the vastus intermedius muscle of the right thigh," Milan said on their website. "The player will be monitored in the coming days in order to better define the extent of the problem and determine the prognosis."
It will be the fourth match in a row that Balotelli will miss for Milan after he was suspended for three games following an aggressive outburst in the aftermath of their 2-1 defeat against Napoli.
Brazilian Kaka, who has been out of action since last month with a thigh injury, has been included in the 21-man squad for Saturday's game at the San Siro.
"He has a maximum of 25 minutes in him," coach Massimiliano Allegri told a news conference on Friday. "It's a gradual recovery and we don't want to risk a relapse."
Milan are languishing in 12th place in Serie A after winning two of their opening seven games.