League One MK Dons defeated visiting Cambridge City 6-1 in a first-round replay on Tuesday to set up a clash with fourth tier Wimbledon, who beat York City 4-3 after extra-time on Monday.
The game will be played on the weekend of December 1.
MK Dons, who retained Wimbledon's 'Dons' nickname when they relocated, returned all the old replica trophies and other memorabilia in 2007 when they declared themselves a new club with no links to the 1988 FA Cup winners.
AFC were formed by fans unhappy at an FA-appointed commission's decision to grant permission to Wimbledon to move almost 50 miles north-west of London to Milton Keynes in 2003.
AFC made rapid progress through the non-league football pyramid, earning promotion in five out of nine seasons before they entered the fourth division in 2011.
On the night, two goals from Shaun Williams combined with efforts from James O'Shea, Dean Bowditch, Dele Ali and Adam Chicksen to set up a first-ever clash with the Dons' London namesakes at stadium:mk; Dave Theobald netted City's consolation.
The Dons controlled the first half, and Williams scored a brilliant goal to break the deadlock after 12 minutes, lobbing keeper Zac Barrett with a dipping left-foot drive from 40 yards.
Ryan Lowe had a shot cleared off the line by Lee Chaffey and O'Shea hit the crossbar before doubling the lead two minutes prior to the break, jinking into the box to curl the ball past Barrett.
Bowditch then made it 3-0 on the stroke of half-time, firing home from a tight angle after Barrett had parried Lowe's fierce shot.
Theobald nodded in Luke Allen's cross from close range on the hour mark to give Cambridge hope but Williams' penalty, Ali's 25-yard screamer and substitute Chicksen's solo goal sealed the rout.
In other first round replays on Tuesday, Barrow defeated Guiseley 1-0 in the only other home victory inside 90 minutes. Garry Hunter was Barrow's last-gasp hero, netting two minutes into injury time.
Hunter crashed home the only goal of the game - a wonder-strike from 20 yards - in the dying moments as the Bluebirds booked themselves a second-round clash at home to Macclesfield.
However it could have all been different had Guiseley substitute Nicky Boshell found the net instead of the bar from six yards as the clocked edged into added time.
In a first half of very few chances, Guiseley had keeper Steve Drench to thank for a fine early save from Richie Baker, but the visitors had chances of their own.
James Walshaw saw an effort tipped over by Barrow goalkeeper Danny Hurst, while Wayne Brooksby, who scored in the 2-2 first-round draw, headed tamely into the arms of Hurst.
Barrow came out with more tempo to their play after the break but it was the Lions who came closest when Danny Ellis headed a free-kick from Danny Boshell narrowly wide.
Walshaw then fired wide from 12 yards after a long clearance from Drench midway through the second half before the late drama.
Tranmere teenager Cole Stockton scored one and created two as the League One leaders finally played and beat non-league Braintree 3-0 to reach the second round.
Ten days after the scheduled fixture was postponed because of heavy rain in Essex, Stockton's 24th-minute volley was only parried skywards by Danny Naisbitt with Joe Thompson bundling home as the ball came down.
Stockton found the net himself after the break, turning and beating Matt Paine before driving home, and he then set up substitute Max Power to make it 3-0 in the dying moments.
Despite looking the better side early on, it was Tranmere and not Braintree who fashioned the best chance but Danny Harrison somehow volleyed over a hanging Thompson cross in the 17th minute. However Thompson made no mistake himself seven minutes later, reacting quickest after Iron goalkeeper Naisbitt could only divert Stockton's strike.
Luke Daley - a replacement for James Mulley in midfield - had a great chance to equalise moments later after a long ball from Naisbitt was not fully cleared, but he fired straight at Rovers keeper Owain fon Williams.
A constant threat throughout, Stockton got the goal he deserved seven minutes after the break and then showed his class just before added time sweeping across the box for Power to fire home himself.
Scott Rendell's double saw Luton progress at the expense of Blue Square Premier League rivals Nuneaton after a 2-0 win.
Hatters striker Rendell took his goal tally for the season to nine with an effort in either half to secure his side's first win in four games - and a tie against Dorchester.
The visitors started brightly and Janos Kovacs' header was cleared off the line by Simon Forsdick after just two minutes.
Luton took the lead on 22 minutes when Rendell touched the ball home from a JJ O'Donnell cross, giving goalkeeper Ben McNamara no chance.
Just before the half-hour mark, Luton were almost pegged back as James Armson fired just wide from a Tom James corner.
Andy Brown then came within inches of an equaliser, but his header from James' free-kick on 38 minutes crashed against the post.
Two minutes before the break, Rendell could have got a second but his effort was easily picked up by keeper McNamara.
But the former Wycombe forward did grab another from the penalty spot on 72 minutes after Gareth Dean brought down Andre Gray.
Morecambe picked up the only goal in a 1-0 success at Rochdale. Gary McDonald's goal on the stroke of half-time helped set up a trip to League One side Coventry.
A dire first half short on incident ended well for the visitors as they snatched the lead two minutes before the break.
Rochdale defender Rhys Bennett was off the pitch receiving treatment following a bang to the head when Izak Reid whipped in a cross.
Lewis Alessandra made the most of the defender's absence, sending a powerful header goalward and, though goalkeeper Josh Lillis was able to parry, he did so into the path of McDonald - who slotted the simplest of finishes home from five yards.
Andrew Fleming should have doubled the lead moments into the second half when he rose unchallenged to meet Alessandra's centre and sent his effort over.
The closest Rochdale came to scoring was a miscued cross from Dele Adebola, Morecambe stopper Barry Roche backpedalling to protect his goal.
Jake Speight scored the decisive penalty which spared Mansfield's blushes against Slough following a 1-1 draw after extra-time of their first-round replay.
The Rebels were the lowest-ranked team left in the competition by the time the clash came round, but Blue Square Bet Premier outfit Mansfield held their nerve - winning 4-1 on spot-kicks after an own goal from Slough captain David Woozley and a Nathan Bowden-Haase header in normal time.
Slough goalkeeper James Warrington made a number of fantastic stops to deny Louis Briscoe in the first half, but could do nothing to stop Mansfield taking the lead through the unfortunate Woozley just before the break.
Lindon Meikle clipped a ball into the box and Exodus Geohaghon's flick-on hit the Slough skipper, who saw the effort loop into his own goal.
The hosts, plying their trade three divisions below Mansfield, equalised through Bowden-Haase, who reacted quickest to Stuart Swift's free-kick which cannoned off the bar.
Mansfield then missed two glorious chances to snatch the game in added time with Speight firing over the bar and Matt Rhead seeing a close-range effort saved by Warrington.
In extra-time, Rhead tamely headed into Warrington's arms before Mansfield captain John Dempster saw red for a lunge on Edward Smith - but it did not prove costly as Speight secured a second-round tie against Lincoln.
Vadaine Oliver was the hero for the Blue Square Premier battlers Lincoln, coming off the bench to score twice in extra-time and hand Walsall a 3-2 replay defeat.
The 20-year-old former Sheffield Wednesday player headed an Alan Power corner firmly into the net 12 minutes into the additional period and intercepted a James Chambers back pass to poke in his second and Lincoln's third two minutes from the end.
Walsall, without a win in 11 games, pulled one back through a deflected Jamie Paterson free-kick in stoppage time, but it was mere consolation.
Lincoln took the lead in the 50th minute when Power beat the offside trap to shoot past David Grof, and the Saddlers thought they had a chance of going through as Richard Taundry fired low into the net from the edge of the box to equalise nine minutes from the end of normal time.
Walsall piled on the pressure but Andrew Boyce was a tower of strength at the heart of the visitors' defence.
Penalty kings Bradford booked their place in the FA Cup second round Northampton on penalties in a dramatic first round replay that finished 3-3 after extra-time at Valley Parade.
All seemed lost for Bradford when Northampton skipper Kelvin Langmead fired visitors into a 3-2 lead in the second period of extra-time, but defender Carl McHugh equalised with seconds remaining to set up a penalty shoot-out, which the home side won 4-2 - their third spot-kick victory this term.
On this occasion Bantams top scorer Nahki Wells was thwarted by Shane Higgs, but failures from Danny East and Langmead left Ricky Ravenhill to complete the triumph from his side's final penalty.
The home side went in front after 35 minutes when James Hanson laid the ball into the path of the unmarked Will Atkinson, who scored with an angled left-footed shot from eight yards, but the visitors equalised two minutes before half-time as Bradford captain Ravenhill was judged to have brought down Ishmel Demontagnac and Demontagnac scored from the spot.
The second half was deadlocked until left-back Joe Widdowson handled substitute Adam Baker's goal bound shot and Wells stepped up to score from 12 yards.
However, when Bradford left back James Meredith failed to cut out a pass from visiting substitute Adebayo Akinfenwa in the third minute of stoppage time, Platt took advantage to score with a low right-footed shot into the far corner to send the tie into extra-time.