In doing so, she denied Norway's Tora Berger for the first time this season the discipline.
Olympic 15km champion Berger was looking to continue her dominance in women's biathlon so far this campaign after a blistering start to the season in Oestersund where she was victorious in all three disciplines.
However she appeared to fade in the final lap in Hochfilzen, in stark contrast to Domracheva, last year’s overall runner-up and a bronze medallist in Vancouver two years ago.
Domracheva eventually claimed the win in 22:24.7, nearly five seconds clear of Finland’s Kaisa Makarainen who grabbed her first podium place of the season.
World Cup leader Berger, who appeared set to miss out on the podium altogether, showed a great deal of spirit to deny her compatriot Synnove Solemdal in fourth as she came home in 22:37.2.
That effort from the Norwegian was enough to limit the damage of missing out on the win and she maintained her lead at the top of the standings with 228 points, still 24 points clear of Domracheva in second.