The 31-year-old Olympic champion, who has won five races this season, claimed her second-lowest finish of the season in the event opener in Italy, placing 14th in the 7.5km sprint.
However the World Cup leader bounced back in style to win the 10km pursuit by almost 19 seconds ahead of Olena Pidhrushina in second and over 25 from Kaisa Makarainen in third.
Berger hit all her targets first time in the range but was well behind early pace setter Makarainen by the time she arrived for her second shoot where she was forced into a penalty loop.
The Norwegian was up to seventh after her third trip to the range, where she again missed a target, yet she made up significant ground to lead at the fourth and final shoot.
Berger led Pidhrushina by 0.2seconds before extending it to 18.9 over the final leg for the win to take her points tally in the overall World Cup standings to 661.
With the second half of the World Cup season now underway Berger leads second-placed Darya Domracheva, who was tenth in the 10km pursuit in Antholz-Anterselva, by 139 points.