Andre Brown had 23 yards on four carries and David Wilson added 16 yards on five attempts for New York.
They were listed as co-starters on the depth chart as the Giants look to replace Ahmad Bradshaw, who was released in the offseason.
Pittsburgh's second-round draft pick Le'Veon Bell, who has been the talk of training camp as he had seemingly moved ahead of Isaac Redman and Jonathan Dwyer, did not play because of a minor knee injury.
Redman, who was listed as number one on the depth chart, ran twice for just seven yards while Dwyer had 15 yards on six carries.
Fourth-stringer LaRod Stephens-Howling, signed as a free agent in the offseason from the Arizona Cardinals, ran for 40 yards on seven carries.
He is considered more of a change-of-pace back who will likely make his biggest impact in the return game.
Both starting quarterbacks were replaced after the first quarter.
New York's Eli Manning completed 2-of-5 passes for one touchdown, a 57-yard strike to Victor Cruz, and Pittsburgh's Ben Roethlisberger was 4-of-8 for 36 yards.
Josh Brown kicked three field goals for the Giants.